Subject: update about l(s)027714
Hi, Dear Sir/Mdm;
Here I update some information about the l(3)s027714 gene(or allele).
this p-element inserts at Blue(bluestreak) (but I can't remember the
exact insertion site as I did the inverse PCR for such a long  time ago
and I didn't keep the sequence result.) And I'm not sure whether the
lethality is indeed caused by this insertion, as I found a second
lethal hit in this line: Dhc64c, for it can't complement the Dhc64C
mutations. So the PlacW inserts at blue gene, and additional lethal
mutation at Dhc64C gene region.
a gentle reminder for people who wants to work on the l(3)sXXXXX line:
this collection more often contains at least one more insertion or
lethal mutation which you have to find out by various mapping, so
people should be very careful when using this collection.
Best regards
Li Zhouhua
Germ Cell Development Group
Temasek Life Sciences Laboratory
1 Research Link
National University of Singapore,
Singapore, 117604
Subject: Re: update about l(s)027714
Dear Li Zhouhua,
The upshot of this is that since there are four genes in the immediate
vicinity of the 027714 insertion it seems premature to assign the
lethality of that insertion to any one of them.  Rescue data or fine
structure deficiency mapping would be required to resolve this problem
properly.  So we will make a note under l(3)S027714 that it may correspond
to blue, but leave the l(3)S027714 record unchanged for now.
I have generated a record for new lethal allele of Dhc64c, named
Dhc64CS027714, and noted that it is separable from
l(3)S027714S027714.
These changes have been attributed to your email, which I've archived as a
personal communication to FlyBase from you.
Thank you for your input.
Rachel
for FlyBase.