Subject: pc for sorting out Exelixis multiples
Personal communication from Bob Levis and Kathy Matthews
Lines submitted by Exelixis to Bloomington with data suggesting
multiple insertions in line:
PBac{PB}CG9619c02710 FBti0041050 (76A3) 
    	Flanking sequence: CZ468157.1
Possible second insertion on 3R?
Data from Exelixis:
10971,"PB","c02710","c02710-3","3R",17786791,"minus","c02710-5","3L",19235486,"minus"
Data from GDP (Levis):
There are two 3prime sequences for this line in the GDP database 
that map to different arms and neither are to the same arm as the 
5prime.
The following 3prime sequence maps to 3R in 93F14:
TTAATCAATTACACT
CGTTTTTGTTTTCACGTATTTATGGGAGGGCAAACGCGTTATAT
The other 3prime sequence maps to 2L-21F1:
TTAAATATTTGCAAG
TGAGGCAATTAGCCAGAATTAGCTTTCAAATAAATATAATATAAATATAT
AATATACAAATATAACATAAGCCATCCATATTTATTATACATTTTACACT
AGTCAAGTGCATTGAGTACTAATATAGTGAATTTACATAACAACCAGTGT
CATAGCGGCATAACCAGCACCACAGAGGCCCACCGATGCGACCTGGCAGT
AACGCAGCTCTTTGCAGATCCGGCGC
STOCK (Bloomington): 
This stock has at least one insertion on chromosome 3 but does 
not have an insertion on chromosome 2.
-------------------------
PBac{PB}GRHRc03956    FBti0041118  (27A1)
	Flanking sequence: CZ468916.1
Possible second insertion on 3R?
Data from Exelixis:
11320,"PB","c03956","c03956-3","3R",5457779,"plus","c03956-5","2L",6706464,"minu
s"
Data from GDP (Levis):
There are two 3prime sequences in the GDP database that map 
to different sites and neither site is near the 5prime flank 
site.
The following sequence maps to SpdS on 3R:
TTAAGCTGTTGAGATTTTAAGGACT
AACCTACAATAGCCTAATCCCATATGTATCCTACATTGAAGGTCCCTAAA
TCATAGATTGTAGAAATCGAAAATTTTCCACTGGTTGTTCAAACACAAAT
TGCCCAGCCAGCATTTTCGGACCCTTTGGTTTTCGTGTGTTTATTCTTGA
TCTGCACCAACAGCCTTATCGATGGAGTCATCTAGGGCCAAAAACGACAG
ACAGAATACTGATAGCAAGTCAGCTCGTCTTTCAGGTTTGAGTTCCTCCA
GTCGAACATTGGGTACACTTGCGC
The other 3prime sequence maps in CG15443 on 2L:
ttatttCAGGTCAATGGTGGTAATCTGAGCCC
GGAATCGATGGATAAACATATTCAGTGGCACCCAGGAACCCGATTTTTCA
TATGTGAATTTATTTTTATACTGGCAAAGAAGTTCGGCGAGGAGATTCAT
TTCCTTTTTGTGCCTTTTTAATTTAATTTTTCTATGATTTCCACGGAAAA
AGTCCAGTGCTCCTTCTGTCCAAAATTATTCACCACTAGCTAGGCGAAAA
TGTGAAAAAACTATACGAAGATAAATGTTTGTTTTTCCAAAGAAATTTAT
TTCAATTTTATATTCTTCAACAGGTAAAGGATATCGATATCCGATAAGAA
TTTTCCCATCAAAACTTGGGCTGACCACCACAGGGCGACCCTTGCAAGTT
TGCGTTTATCTCAGCTGTTAAATATGGTAAACACACCTTAGTTTCAAAAA
TCATCATTTTGATAAATAATTTGAATTTCGAACATCGATAATAAATCGAT
TTAGCATATGGCCCAAAGAATAGGGTGCCCAGTGTACTGTGCACAATGTG
AAGTTTAGCTTATTATTATTTTGAATTGTTCATCAAGGCAATCGCATTAA
CTTTCAATTAATTGGGATGTAT
STOCK (Bloomington):
This stock has at least one insertion on 2 but does
not have an insertion on chromosome 3.
---------------------------------------------------
PBac{PB}deflc06100  FBti0041269 (67C5)
	Flanking sequence: CZ470496.1
Possible second insertion on 4?
Data from Exelixis:
17746,"PB","c06100","c06100-3","3L",9681120,"plus","c06100-5","4",1050811,"plus"
Data from GDP (Levis):
There are three 5prime sequences in the GDP database.  Two of 
these map to the same site as the 3prime flank and one maps to 
CG11076 on the 4th chromosome
1) The following sequence maps to defl on 3L:
TTAAGGAAGCTCTCGTTGAAAGTGCTCACGCGGGTGCCGGTCAGGTGAGAC
ATGGTTACAGGTTTCCTCACTCACATTTATCGCAGATAAATCGTGTTTAT
TGAGGTTTTCATTAGCCAAACGACACAAAAACAAACCGGCTGCAAATGCT
TCTTCTTGCTAATTTGTCGTTCGTAGGAAACTCCGG
2) The following sequence maps to CG11076 in 102F5:
ttaa
atcctatctagcTTTCGTAGTTCCTGtgatc
3) The following sequence maps to the same site in defl as #1
TTAAGGAAGCTCTCGTTGAAAGTGCTCACGCGGGTGCCGGTCAGGTGAGACA
TGGTTACAGGTTTCCTCACTCACATTTATCGCAGATAAATCGTGTTTATT
GAGGTTTTCATTAGCCAAACGACACAAAAACAAACCGGCTGCAAATGCTT
CTTCTTGCTAATTTGTCGTTCGTAGGAAACTCCGGTATCATTGCAGCGAT
AGCTGGTCACTTAACAGTACAAAAACGATTGAATCCAAAGTCTATAATGT
CAATTCTTAAGAAGTGTTTATTAAGATC
STOCK (Bloomington):
This stock has an insertion on chromosome 3, but does
not have an insertion on chromosome 4.
FlyBase comment: "4th chromosome" read is only 35nt and
maps to an INE-1 element.  There may be a second insertion,
but cannot determine where it maps.
--------------------------------------
c05504 -- flanking sequence data (CZ470048.1, CZ470049.1) 
support 2 insertions.
Existing: PBac{PB}Irisc05504  FBti0041227  (21F1)
     (Misnamed; misses Iris)
	Flanking sequence: CZ470049.1
A second insertion:  PBac{PB}c05504b (3h)
	Flanking sequence: CZ470048.1
STOCK (Bloomington):
This stock contains only an insertion on chromosome 3.