Subject: personal communication regarding fs(1)M3+t8
I have obtained permission from Dr Jimenez to curate this message as a
personal communication.  fs(1)ph is a synonym for fs(1)M3.
Sian
-----Forwarded Message-----
Subject: Re: fs(1)ph '{'AKA fs(1)M3'}'
Dear Dr. Gramates,
Thank you for your message. The sequences of the primers used to generate
the fs(1)ph rescue construct are as follows:
Primer 1: 5'-ctagctgcgtgactcactcg-3'
Primer 2: 5'-ccttcgaggacgtggctagc-3'
The transformation plasmid was made by digesting the PCR product with
Eco47III and NheI, and cloning the resulting 8-kb fragment in pCaSpeR4
digested with HpaI and XbaI.
Please do not hesitate to contact me again if you need further
clarifications.
With best wishes,
Gerardo Jimenez
Institut de Biologia Molecular de Barcelona-CSIC
Parc Cientafic de Barcelona
Josep Samitier, 1-5
08028 Barcelona, Spain
> Dear Dr Jimenez
> 
> I am a curator for FlyBase, and am curating the genomic coordinates of
> rescue constructs.  In particular, I am curating the rescue constructs
> from your paper, Jimenez, et al, 2002 (Genes and Development
>  16:913-918 ).
> 
> The 8 kb rescue fragment for fs(1)ph is described as having been
> PCR-generated from genomic DNA.  Would it be possible for you to send me
> the the sequence of the primers used to generate this rescue fragment?
> 
> Sincerely,
> Sian Gramates, FlyBase