Subject: Re: Question about Act88F mutant
Sian,
This mutation was made by PCR mutagenesis in the wild type cloned Act88F 
gene. Mutated gene was inserted into the Carnegie 20 (Pry+) vector and 
transfected into a ry506 Act88FKM88 es background. (KM88 is the 
null mutation in the Act88F gene). The work was done by Dr Stephan 
Schmitz in my lab and the details are in his PhD thesis (University of 
York 1999) 'Mutational analysis of proposed myosin binding sites on actin'.
I hope that is the information you require. If you need further data I 
can probably supply it.
John
L. Sian Gramates wrote:
> Dear Dr Sparrow,
> 
> I am a curator for FlyBase, and am curating the genomic coordinates of
> several Act88F mutants.  A paper from your lab, Nongthomba, et al., 2003
> (Genetics  164:209-222 ) is the only curated reference we have for
> Act88FE334Q. Would it be possible for you to give us a bit more
> information about this mutant, including the original source and
> mutagen?  I am also trying to identify the sequence change in this
> mutant. The name seems to suggest a substitution of Gln for 334Glu;
> could you confirm that?
> 
> Sincerely,
> Sian Gramates, FlyBase
-- 
Prof John C. Sparrow
Department of Biology (Area 10)
University of York
York YO10 5DD (UK)