Isolation and characterization of Df(3L)BSC219
Jill Gresens and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC219 was isolated as a FLP recombinase-induced recombination event involving P{XP}d06673 and PBac{WH}f06950. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; P{XP}d06673/P{XP}f06950 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC219 from the segment of P{XP}d06673 to the left of its FRT site and the segment of P{XP}f06950 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3L)BSC219 predicted from the Release 4 genomic coordinates of the transposable element insertions sites using are 66D4;66D6. Df(3L)BSC219 failed to complement foij8E8.