Isolation and characterization of Df(3L)BSC170
Jill Gresens, Rachel Andrade and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC170 was isolated as a FLP recombinase-induced recombination event involving P{XP}Argkd07847 and PBac{WH}Rdlf02994. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, w1118; P{XP}Argkd07847/ PBac{WH}Rdlf02994 females crossed to w1118; P{w+mC=hs-hid}2, wgSp-1/CyO males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC170 from the segment of P{XP}Argkd07847 to the left of its FRT site and the segment of PBac{WH}f02994 to the right of its FRT site. The cytological breakpoints of Df(2L)BSC170 predicted from the transposable element insertions sites using Release 4 are 66F4;67A1. It failed to complement bol1 and Rdl1.