Isolation and characterization of Df(2L)BSC290
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC290 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f02059 and P{XP}d08229. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; PBac{WH}f02059/P{XP}d08229 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC290 from the segment of PBac{WH}f02059 to the left of its FRT site and the segment of P{XP}d08229 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}d08229 to be at Release 3 genomic coordinate 13049022 on chromosome arm 2L. This corresponds to 34A5 on the Release 5 genome map. The predicted position of PBac{WH}f02059 on the Release 5 map is 33F2 . Consequently, the cytological breakpoints of Df(2L)BSC290 are predicted to be 33F2;34A5. It failed to complement Vha68-2s4214 and Df(2L)ED778