Isolation and characterization of Df(3R)BSC174
Jill Gresens and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC174 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f00382 and P{XP}CG31531d00921. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6B, Tb1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}f00382/P{XP}CG31531d00921 males. The males were heat shocked as larvae as described in Parks et al. Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC174 from the segment of PBac{WH}f00382 to the left of its FRT site and the segment of P{XP}CG31531d00921 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3R)BSC174 predicted from the transposable element insertions sites using Release 4 coordinates are 82C1;82D1. It failed to complement Karybeta3j3A4.