Isolation and characterization of Df(2L)BSC225
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC225 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f07515 and P{XP}d10451. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; PBac{WH}f07515/P{XP}d10451 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC225 from the segment of PBac{WH}f07515 to the left of its FRT site and the segment of P{XP}d10451 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}d10451 to be Release 3 genomic coordinate 4813677 on chromosome arm 2L. This corresponds to 25A7 on both the Release 3 and Release 4 genome maps. The predicted position of PBac{WH}f07515 on the Release 4 map is 25A3. Consequently, the cytological breakpoints of Df(3R)BSC225 are predicted to be 25A3;25A7. It failed to complement Df(2L)ED250, but complemented Df(2L)Exel6010.