Isolation and characterization of Df(3L)BSC249
Stacey Christensen, Jill Gresens and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC249 was isolated as a FLP recombinase-induced recombination event involving P{XP}RpLP0d09567 and PBac{WH}olf413f02553. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; P{XP}RpLP0d09567/PBac{WH}olf413f02553 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC249 from the segment of P{XP}RpLP0d09567to the left of its FRT site and the segment of PBac{WH}olf413f02553 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3L)BSC249 predicted from the transposable element insertions sites using Release 4 are 79B2;79D2. The presence of a deletion was confirmed cytologically, though the breakpoints were not analyzed in detail.