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Christensen, S., Cook, K. (2007.3.22). Isolation and characterization of Df(2R)BSC260. 
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FBrf0199246
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Personal communication to FlyBase
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Date: Thu, 22 Mar 2007  14:52:18  -0400
To: flybase-updates@XXXX
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(2R)BSC260
Cc: Stacey Christensen <sjchristXXXX>, mdealXXXX, kaufman@XXXX
Isolation and characterization of Df(2R)BSC260
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC260 was isolated as a FLP recombinase-induced recombination 
event involving PBac{WH}f03311 and P{XP}vimar[d00911]. The deletion 
was isolated as a chromosome lacking miniwhite markers in progeny of 
P{hsFLP}1, y[1] w[1118]; PBac{WH}f03311/P{XP}vimar[d00911] males 
crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males 
were heat shocked as larvae as described in Parks et al., Nature 
Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in 
preceding and succeeding generations maintained the original genetic 
background of the Exelixis insertion stocks (Thibault et al., Nature 
Genetics 36: 283-287, 2004; FBrf0175002). The recombination event 
generated the genetic element P+PBac{XP5.WH5}BSC260 from the segment 
of PBac{WH}f03311 to the left of its FRT site and the segment of 
P{XP}vimar[d00911] to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
The cytological breakpoints of Df(2R)BSC260 predicted from the 
Release 4 genomic coordinates of the transposable element insertions 
sites are 42C3;42E1. It failed to complement Eb1[04524] and Df(2R)ED1770.
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX
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    English
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    Aberrations (2)
    Alleles (1)
    Genes (1)
    Insertions (3)
    Transgenic Constructs (1)