Isolation and characterization of Df(3L)BSC282
Stacey Christensen, Jill Gresens and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC282 was isolated as a FLP recombinase-induced recombination event involving P{XP}d08188 and PBac{WH}dpr6f07173. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; P{XP}d08188/PBac{WH}dpr6f07173 males. The males were heat shocked as larvae as described in Parks et al. Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC282 from the segment of P{XP}d08188 to the left of its FRT site and the segment of PBac{WH}dpr6f07173 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3L)BSC282 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 67C7;67D5. Df(3L)BSC282 failed to complement CG8108EY14316 and Df(3L)AC1.