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Christensen, S., Cook, K., Cook, K. (2007.10.29). Isolation and characterization of Df(2R)BSC314. 
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From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updatesXXXX, Stacey Christensen <sjchristXXXX>
Subject: 	Isolation and characterization of Df(2R)BSC314
Date: 	Mon, 29 Oct 2007  12:07:15  -0400  ( 16:07  GMT)
Isolation and characterization of Df(2R)BSC314
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC314 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}CG30015[d06273] and PBac{RB}e01872. The deletion 
was isolated as a chromosome lacking miniwhite markers in progeny of 
P{hsFLP}1, y[1] w[1118]; P{XP}CG30015[d06273]/PBac{RB}e01872 males 
crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males 
were heat shocked as larvae as described in Parks et al., Nature 
Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in 
preceding and succeeding generations maintained the original genetic 
background of the Exelixis insertion stocks (Thibault et al., Nature 
Genetics 36: 283-287, 2004; FBrf0175002). The recombination event 
generated the genetic element P+PBac{XP5.RB3}BSC314 from the segment 
of P{XP}CG30015[d06273] to the left of its FRT site and the segment 
of PBac{RB}e01872 to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' 
for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in 
the Supplementary Methods. The cytological breakpoints of 
Df(2R)BSC314 predicted from the Release 5 genomic coordinates of the 
transposable element insertions sites are 47C5;47D4. It failed to 
complement CG7712[KG05594], Df(2R)Exel6059 and Df(2R)ED2155. 
Df(2R)FDD-0077658 is a synonym for Df(2R)BSC314.
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology 
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX 
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    Aberrations (3)
    Alleles (1)
    Genes (1)
    Insertions (3)
    Transgenic Constructs (1)