Isolation and characterization of Df(2R)BSC328
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC328 was isolated as a FLP recombinase-induced recombination event involving P{XP}d02995 and PBac{WH}CG12384f06686. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}d02995/PBac{WH}CG12384f06686 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC328 from the segment of P{XP}d02995 to the left of its FRT site and the segment of PBac{WH}CG12384f06686 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2R)BSC328 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 47C1;47F5. It failed to complement CG7712KG05594 and shn1.