Isolation and characterization of Df(3L)BSC372
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC372 was isolated as a FLP recombinase-induced recombination event involving P{XP}d08991 and PBac{WH}CG5150f05027. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; P{XP}d08991/PBac{WH}CG5150f05027 males. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC372 from the segment of P{XP}d08991 to the left of its FRT site and the segment of PBac{WH}CG5150f05027 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3L)BSC372 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 64C4;64D5. Df(3L)BSC372 failed to complement Klp64Dk1 and sinu06524.