Isolation and characterization of Df(3R)BSC318
Stacey Christensen, Jill Gresens, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC318 was isolated as a FLP recombinase-induced recombination event involving P{XP}CG3744d09029 and PBac{WH}CG11836f02631. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; P{XP}CG3744d09029/PBac{WH}CG11836f02631 males. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC318 from the segment of P{XP}CG3744d09029 to the left of its FRT site and the segment of PBac{WH}CG11836f02631 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3R)BSC318 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 96B2;96B20. Df(3R)BSC318 failed to complement ssh01207.