Isolation and characterization of Df(3L)BSC394
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC394 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f01812 and P{XP}nudEd10518. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}f01812/P{XP}nudEd10518 males. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC394 from the segment of PBac{WH}f01812 to the left of its FRT site and the segment of P{XP}nudEd10518 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3L)BSC394 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 67C9;67D1. Df(3L)BSC394 failed to complement Gap1B2. Df(3L)FDD-0279140 is a synonym for Df(3L)BSC394.