Isolation and characterization of Df(2R)BSC338
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC338 was isolated as a FLP recombinase-induced recombination event involving P{XP}olf186-Fd06331 and PBac{WH}f02733. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}olf186-Fd06331/PBac{WH}f02733 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC338 from the segment of P{XP}olf186-Fd06331 to the left of its FRT site and the segment of PBac{WH}f02733 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2R)BSC338 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 54F1;55B1 . It failed to complement thr1 and Df(2R)PC4. Df(2R)FDD-0078341 is a synonym for Df(2R)BSC338.