Isolation and characterization of Df(2R)BSC355
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC355 was isolated as a FLP recombinase-induced recombination
event involving P{XP}CG14478d09297 and PBac{WH}eIF3-S9f05638. The
deletion was isolated as a chromosome lacking miniwhite markers in
progeny of P{hsFLP}1, y1 w1118;
P{XP}CG14478d09297/PBac{WH}eIF3-S9f05638 males crossed to
w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat
shocked as larvae as described in Parks et al., Nature Genetics 36:
288-292, 2004 (FBrf0175003). This cross and crosses in preceding and
succeeding generations maintained the original genetic background of
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36:
283-287, 2004; FBrf0175002). The recombination event generated the
genetic element P+PBac{XP5.WH5}BSC355 from the segment of
P{XP}CG14478d09297 to the left of its FRT site and the segment of
PBac{WH}eIF3-S9f05638 to the right of its FRT site. Its presence
was verified using the PCR methods and primers described in Parks et
al. The cytological breakpoints of Df(2R)BSC355 predicted from the
Release 5 genomic coordinates of the transposable element insertions
sites are 54B16;54C3. The presence of a deletion was confirmed
cytologically, though the breakpoints were not analyzed in detail. It
failed to complement Df(2R)BSC161.