Isolation and characterization of Df(3R)BSC176
Stacey Christensen, Jill Gresens, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC176 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}katanin-60f05910 and P{XP}Hphd03981. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6B, Tb1 females crossed to P{hsFLP}1, w1118; PBac{WH}katanin-60f05910/P{XP}Hphd03981 males. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC176 from the segment of PBac{WH}katanin-60f05910 to the left of its FRT site and the segment of P{XP}Hphd03981 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3R)BSC176 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 82F6;82F8. Df(3R)BSC176 failed to complement CG12163EY04405.