Isolation and characterization of Df(3L)BSC368
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC368 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f01986 and P{XP}CG11593d06001. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}f01986/P{XP}CG11593d06001 males. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC368 from the segment of PBac{WH}f01986 to the left of its FRT site and the segment of P{XP}CG11593d06001 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of PBac{WH}f01986 to be at Release 3 genomic coordinate 3740450 on chromosome arm 3L. This corresponds to 63F1 on the Release 3 and 5genome map. The predicted position of P{XP}CG11593d06001 on the Release 5 map is 64A4. Consequently, the cytological breakpoints of Df(3L)BSC368 are predicted to be 63F1;64A4. Df(3L)BSC368 failed to complement Awh63Ea-1 and Sc21. Df(3L)FDD-0283239 is a synonym for Df(3L)BSC368.