ate: Mon, 29 Oct 2007  12:08:32  -0400
Isolation and characterization of Df(3L)BSC378
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC378 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f05450 and P{XP}d07141. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}f05450/P{XP}d07141 males. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC378 from the segment of PBac{WH}f05450 to the left of its FRT site and the segment of P{XP}d07141 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3L)BSC378 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 67E7;68A6. Df(3L)BSC378 failed to complement tnarI075. Df(3L)FDD-0360171 is a synonym for Df(3L)BSC378.