The following information accompanied stocks donated to the Bloomington Stock Center by Jieya Shao and Marc Diamond, University of California at San Francisco.
P{UAS-Hsap\PFN1.D} is a P{UAST} construct of Human Profilin 1 (PFN1).
P{UAS-Hsap\PFN1.D}2 is a homozygous viable and fertile, second chromosome insertion.
P{UAS-Hsap\PFN1.S137A} is a similar construct encoding a mutant PFN1 protein with a S137A amino acid substitution.  Likewise, P{UAS-Hsap\PFN1.S137D} encodes PFN1 with a S137D substitution.
P{UAS-Hsap\PFN1.S137A}2 is a homozygous viable and fertile, second chromosome insertion.
P{UAS-Hsap\PFN1.S137A}3 and P{UAS-Hsap\PFN1.S137D}3 are homozygous viable and fertile, third chromosome insertions.
Neither the S137A nor S137D substitutions affect the binding of PFN1 to actin as assayed by immunoprecipitation.  The S137D protein variant does not bind huntingtin, whereas the S137A variant does.