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FlyBase ID
FBrf0204179
Publication Type
Research paper
Abstract
One major limit to analysis of developmental processes is the ability to isolate enough material of a specific age, genotype, or other relevant characteristic. Flow cytometry-based cell sorting instruments are capable of isolating near pure populations of individual cells based on size and fluorescence signals. The COPAS Select and Express flow cytometry instruments are able to analyze and sort intact Drosophila embryos or larva based on similar characteristics. By combining this technology with an appropriate GFP transgene or other fluorescent marker, one can isolate nearly pure populations based on a variety of characteristics, including sex.