Isolation and characterization of Df(2R)BSC433
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC433 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG6967f07741 and P{XP}GstS1d07643. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; PBac{WH}CG6967f07741/P{XP}GstS1d07643 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC433 from the segment of PBac{WH}CG6967f07741 to the left of its FRT site and the segment of P{XP}GstS1d07643 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2R)BSC433 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 53F4;53F8.