Isolation and characterization of Df(2L)BSC456
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC456 was isolated as a FLP recombinase-induced recombination event involving P{XP}d01181 and PBac{WH}CG3639f01300. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}d01181/PBac{WH}CG3639f01300 males crossed to w1118; P{hs-hid}2, wgSp-1/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC456 from the segment of P{XP}d01181 to the left of its FRT site and the segment of PBac{WH}CG3639f01300 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}d01181 to be Release 3 genomic coordinate 481002 on chromosome arm 2L. The Gene Disruption project determined the insertion site of P{XP}d01181 to be Release 3 genomic coordinate 481354 on arm 2L. This corresponds to 21C6 on the Release 3 genome map and 21D1 on the Release 5 genome map. The predicted position of PBac{WH}CG3639f01300 on the Release 5 map is 21E2. Consequently, the cytological breakpoints of Df(2L)BSC456 are predicted to be 21D1;21E2. Df(2L)BSC456 failed to complement Hsp60B06619 and Df(2L)BSC107.