Isolation and characterization of Df(3L)BSC412
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC412 was isolated as a FLP recombinase-induced recombination event involving P{XP}d01275 and PBac{WH}Mrtff02572. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; P{XP}d01275/PBac{WH}Mrtff02572 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC412 from the segment of P{XP}d01275 to the left of its FRT site and the segment of PBac{WH}Mrtff02572 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3L)BSC412 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 62E8;62F3. Df(3L)BSC412 failed to complement Df(3L)Exel6091 and Df(3L)BSC23.