Isolation and characterization of Df(3R)BSC500
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC500 was isolated as a FLP recombinase-induced recombination event involving P{XP}d00762 and PBac{WH}CAP-D2f03381. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; P{XP}d00762/PBac{WH}CAP-D2f03381 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC500 from the segment of P{XP}d00762 to the left of its FRT site and the segment of PBac{WH}CAP-D2f03381 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}d00762 to be Release 3 genomic coordinate 24927615 on chromosome arm 3R. This corresponds to 98F10 on the Release 3 and Release 5 genome maps. The predicted position of PBac{WH}CAP-D2f03381 on the Release 5 map is 99B7. Consequently, the cytological breakpoints of Df(3R)BSC500 are predicted to be 98F10;99B7. Df(3R)BSC500 failed to complement stg4.