Isolation and characterization of Df(2L)BSC481
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC481 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG4764f04578 and P{XP}d08273. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; PBac{WH}CG4764f04578/P{XP}d08273 males crossed to w1118; P{hs-hid}2, wgSp-1/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC481 from the segment of PBac{WH}CG4764f04578 to the left of its FRT site and the segment of P{XP}d08273 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2L)BSC481 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 21F1;21F2. Df(2L)BSC481 failed to complement capt06955 and Tango14DG04806.