Isolation and characterization of Df(3R)BSC421
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC421 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f08107 and P{XP}CG1090d08005. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}f08107/P{XP}CG1090d08005 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC421 from the segment of PBac{WH}f08107 to the left of its FRT site and the segment of P{XP}CG1090d08005 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of PBac{WH}f08107 to be Release 3 genomic coordinate 77490 on chromosome arm 3R. This corresponds to 82A1 on the Release 3 and Release 5 genome maps. The predicted position of P{XP}CG1090d08005 on the Release 5 map is 82A4. Consequently, the cytological breakpoints of Df(3R)BSC421 are predicted to be 82A1;82A4. Df(3R)BSC421 failed to complement hkbA321R1 and Df(3R)ED5020.