Isolation and characterization of Df(2L)BSC453
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC453 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG31718f06333 and P{XP}d08708. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; PBac{WH}CG31718f06333/P{XP}d08708 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC453 from the segment of PBac{WH}CG31718f06333 to the left of its FRT site and the segment of P{XP}d08708 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}d08708 to be Release 3 genomic coordinate 10450004 on chromosome arm 2L. This corresponds to 31E5 on the Release 3 and Release 5 genome map. The predicted position of PBac{WH}CG31718f06333 on the Release 5 map is 31E1. Consequently, the cytological breakpoints of Df(2L)BSC453 are predicted to be 31E1;31E5. It failed to complement pie54 and RpS27Amfs31.