Isolation and characterization of Df(2L)BSC454
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC454 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG3645f02260 and P{XP}d10144. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; PBac{WH}CG3645f02260/P{XP}d10144 males crossed to w1118; P{hs-hid}2, wgSp-1/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC454 from the segment of PBac{WH}CG3645f02260 to the left of its FRT site and the segment of P{XP}d10144 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}d10144  to be Release 3 genomic coordinate 307085 on chromosome arm 2L. This corresponds to 21B8 on the Release 3 and Release 5 genome map. The predicted position of PBac{WH}CG3645f02260 on the Release 5 map is 21B7. Consequently, the cytological breakpoints of Df(2L)BSC454 are predicted to be 21B7;21B8. Df(2L)BSC454 failed to complement U2af3806751.