Subject: 	FB Help Mailer: 205 Personal communication
 comments:    Dear Flybase,
    It has been brought to my attention that the translocation T(1;Y)B146 is listed as an allele of mud.
    http://flybase.bio.indiana.edu/reports/FBal0028449.html
    I would like to share with you data that I have collected which suggests that the translocation breakpoint is not within the mud gene.  
    Several years ago as part of a mapping project in the lab of Howard Nash, I probed salivary gland chromosomes of T(1;Y)B146 flies with labeled Tamkun library cosmids cos99 and cos57.  I found both of these probes to give signal in the distal portion of the X which is fused to the Y centromere.  Sequencing the ends of both of these cosmids locates them centromere proximal to mud.  Taken in its entirety, the breakpoint in T(1;Y)B146 is not within the mud gene, but located at least 150kb centromere proximal to mud.
    I hope this information is of some use, and please contact me if you have any questions.
    Sincerely,
    Robert
 realname: Robert Scott