Subject: 	Isolation and characterization of Df(3R)BSC525
Isolation and characterization of Df(3R)BSC525
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC525 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}MTA1-liked09140 and PBac{WH}CG2017f01712. 
The deletion was isolated as a chromosome lacking miniwhite markers 
in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 females crossed 
to P{hsFLP}1, y1 w1118; 
P{XP}MTA1-liked09140/PBac{WH}CG2017f01712 males. The males were 
heat shocked as larvae as described in Parks et al., Nature Genetics 
36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding 
and succeeding generations maintained the original genetic background 
of the Exelixis insertion stocks (Thibault et al., Nature Genetics 
36: 283-287, 2004; FBrf0175002). The recombination event generated 
the genetic element P+PBac{XP5.WH5}BSC525 from the segment of 
P{XP}MTA1-liked09140 to the left of its FRT site and the segment of 
PBac{WH}CG2017f01712 to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
The cytological breakpoints of Df(3R)BSC525 predicted from the 
Release 5 genomic coordinates of the transposable element insertions 
sites are 83B7;83C5. Df(3R)BSC525 failed to complement sec23j13C8 
and casj1C2.