Isolation and characterization of Df(1)BSC536
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(1)BSC536 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CBPf00211 and P{XP}d01377. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of PBac{WH}CBPf00211/P{XP}d01377; MKRS, P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC536 from the segment of PBac{WH}CBPf00211 to the left of its FRT site and the segment of P{XP}d01377 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(1)BSC536 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 7B2;7C1. It failed to complement ct6.