Isolation and characterization of Df(3L)BSC557
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC557 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG10674f05385 and P{XP}Blimp-1d06859. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}CG10674f05385/P{XP}Blimp-1d06859 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC557 from the segment of PBac{WH}CG10674f05385 to the left of its FRT site and the segment of P{XP}Blimp-1d06859 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3L)BSC557 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 67C7;64E1. Df(3L)BSC557 failed to complement sinu06524 and Klp64Dk1.