Isolation and characterization of Df(3R)BSC565
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC565 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}beat-IIaf00670 and P{XP}cpod05694. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}beat-IIaf00670/P{XP}cpod05694 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC565 from the segment of PBac{WH}beat-IIaf00670 to the left of its FRT site and the segment of P{XP}cpod05694 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of PBac{WH}beat-IIaf00670 to be at Release 3 genomic coordinate 13181235 on chromosome arm 3R. This corresponds to 90A2 on the Release 3 and Release 5 genome maps. The predicted position of P{XP}cpod05694 on the Release 5 map is 90D1. Consequently, the cytological breakpoints of Df(3R)BSC565 are predicted to be 90A2;90D1. Df(3R)BSC565 failed to complement l(3)0788207882 and Df(3R)ED5780.