Isolation and characterization of Df(3R)BSC573
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC573 was isolated as a FLP recombinase-induced recombination event involving P{XP}d03390 and PBac{WH}CG14545f05409. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; P{XP}d03390/PBac{WH}CG14545f05409 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC573 from the segment of P{XP}d03390 to the left of its FRT site and the segment of PBac{WH}CG14545f05409 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3R)BSC573 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 96F2;96F3. The presence of a deletion was confirmed cytologically, though the breakpoints were not analyzed in detail.