Chan, W.M., Shaw, P.C., Chan, H.Y.E. (2007). A green fluorescent protein-based reporter for protein nuclear import studies in Drosophila cells.  Fly 1(6): 340--342.

FBrf0205056

Research paper

Green fluorescent protein-based reporters are commonly used to investigate protein nucleocytoplasmic transport. In this study we developed a novel reporter GFP2-GST which consists of two copies of GFP and one copy of GST, and tested it in two commonly used Drosophila cell lines. The size of the GFP2-GST reporter exceeds the passive diffusion limit across the nuclear pore complexes. It shows an exclusive cytoplasmic localization and displays a restrictive nuclear localization when a nuclear localization signal is appended. This reporter will largely facilitate the characterization and identification of NLS sequences in the fly proteome.