Isolation and characterization of Df(3L)BSC612
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC612 was isolated as a FLP recombinase-induced recombination event involving P{XP}d01126 and PBac{WH}dallyf01097. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; P{XP}d01126/PBac{WH}dallyf01097 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC612 from the segment of P{XP}d01126 to the left of its FRT site and the segment of PBac{WH}dallyf01097 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3L)BSC612 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 66D10;66E1. Df(3L)BSC612 failed to complement h22.