Isolation and characterization of Df(1)BSC623
Kim Cook, Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(1)BSC623 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f01428 and P{XP}d00754. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of PBac{WH}f01428/P{XP}d00754; MKRS, P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC623 from the segment of PBac{WH}f01428 to the left of its FRT site and the segment of P{XP}d00754 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(1)BSC623 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 11A9;11E1. It failed to complement Tis11G0124 and SmrG0361.