Isolation and characterization of Df(1)BSC642
Kim Cook, Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(1)BSC642 was isolated as a FLP recombinase-induced recombination event involving P{XP}CG8289d10824 and PBac{WH}mnbf01371. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{XP}CG8289d10824/PBac{WH}mnbf01371; MKRS, P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC642 from the segment of P{XP}CG8289d10824 to the left of its FRT site and the segment of PBac{WH}mnbf01371 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(1)BSC642 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 16B10;16E4. The presence of a deletion was confirmed cytologically, though the breakpoints were not analyzed in detail.