Isolation and characterization of Df(3R)BSC684
Kim Cook, Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC684 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG5863f05947 and P{XP}d10217. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}CG5863f05947/P{XP}d10217 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC684 from the segment of PBac{WH}CG5863f05947 to the left of its FRT site and the segment of P{XP}d10217 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3R)BSC684 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 90A6;90B7. Df(3R)BSC684 failed to complement l(3)0788207882 with a partial lethality phenotype; escapers were female sterile.