Isolation and characterization of Df(2R)BSC701
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC701 was isolated as a FLP recombinase-induced recombination event involving PBac{RB}CG9143e00691 and P{XP}d02039. The deletion was isolated as a chromosome carrying two copies of the miniwhite marker in progeny of P{hsFLP}1, y1 w1118; PBac{RB}CG9143e00691/P{XP}d02039 males crossed to w1118; P{hs-hid}2, wgSp-1/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP3.RB5}BSC701 from the segment of PBac{RB}CG9143e00691 to the left of its FRT site and the segment of P{XP}d02039 to the right of its FRT site. The cytological breakpoints of Df(2R)BSC701 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 56F15;57A9. Df(2R)BSC701 failed to complement Df(2R)BSC400 and Df(2R)Exel7164.