Isolation and characterization of Df(1)BSC761
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(1)BSC761 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f04029 and P{XP}crld03572. The deletion was isolated as a chromosome carrying two copies of the miniwhite marker in progeny of PBac{WH}f04029/P{XP}crld03572; MKRS, P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP3.WH3}BSC761 from the segment of PBac{WH}f04029 to the left of its FRT site and the segment of P{XP}crld03572 to the right of its FRT site. Exelixis, Inc. determined the insertion site of PBac{WH}f04029 to be Release 3 genomic coordinate 16194176 on the X chromosome. This corresponds to 14D1 on the Release 3 and Release 5 genome maps. The predicted position of P{XP}crld03572 on the Release 5 map is 14F1. Consequently, the cytological breakpoints of Df(1)BSC761 are predicted to be 14D1;14F1. The presence of a deletion was confirmed cytologically, though the breakpoints were not analyzed in detail.