Isolation and characterization of Df(3L)BSC732
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC732 was isolated as a FLP recombinase-induced recombination event involving P{XP}d02504 and PBac{WH}DNApol-alpha50f02992. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to P{hsFLP}1, y1 w1118; P{XP}d02504/PBac{WH}DNApol- alpha50f02992 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC732 from the segment of P{XP}d02504 to the left of its FRT site and the segment of PBac{WH}DNApol- alpha50f02992 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}d02504 to be Release 3 genomic coordinate 7828494 on chromosome arm 3L. The Gene Disruption project determined the insertion site of P{XP}d02504 to be Release 3 genomic coordinate 7828548 on arm 3L. This corresponds to 66A17 on the Release 3 and Release 5 genome maps. The predicted position of PBac{WH}DNApol- alpha50f02992 on the Release 5 map is 66C8. Consequently, the cytological breakpoints of Df(3L)BSC732 are predicted to be 66A17;66C8. Df(3L)BSC732 failed to complement Nmtj1C7.