Isolation and characterization of Df(1)BSC772
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(1)BSC772 was isolated as a FLP recombinase-induced recombination event involving PBac{RB}CG3632e04331 and P{XP}d01564. The deletion was isolated as a chromosome carrying two copies of the miniwhite marker in progeny of PBac{RB}CG3632e04331/P{XP}d01564; MKRS, P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP3.RB5}BSC772 from the segment of PBac{RB}CG3632e04331 to the left of its FRT site and the segment of P{XP}d01564 to the right of its FRT site. The cytological breakpoints of Df(1)BSC772 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 14B9;14C4. Df(1)BSC772 failed to complement mei-41RT1. The presence of a deletion was confirmed cytologically, though the breakpoints were not analyzed in detail.