Isolation and characterization of Df(2R)BSC786
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC786 was isolated as a FLP recombinase-induced recombination event involving P{XP}d08960 and PBac{RB}CG2921e02644. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}d08960/PBac{RB}CG2921e02644 males crossed to w1118; P{hs-hid}2, wgSp-1/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.RB3}BSC786 from the segment of P{XP}d08960 to the left of its FRT site and the segment of PBac{RB}CG2921e02644 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. with the substitution of the primer 5'-GCTTCTAAACGCTTACGCATAAACGATG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods. The breakpoints of Df(2R)BSC786 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  2R:17549804 ;17963253 and the cytological breakpoints predicted from these coordinates are 57F6;58B3. Df(2R)BSC786 failed to complement Df(2R)ED3923 and Df(2R)Exel6077.