Isolation and characterization of Df(3R)BSC806
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC806 was isolated as a FLP recombinase-induced recombination event involving PBac{RB}e01987 and P{XP}d00762. The deletion was isolated as a chromosome carrying two copies of the miniwhite marker in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to P{hsFLP}1, y1 w1118; PBac{RB}e01987/P{XP}d00762 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP3.RB5}BSC806 from the segment of PBac{RB}e01987 to the left of its FRT site and the segment of P{XP}d00762 to the right of its FRT site. Exelixis, Inc. determined the insertion site of P{XP}d00762 to be Release 3 genomic coordinate 24927615 on chromosome arm 3R. This corresponds to Release 5 coordinate  3R:24938249 . The insertion site of PBac{RB}e01987 is Release 5 coordinate  3R:24696033 . Consequently, the breakpoints of Df(3R)BSC806 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  3R:24696033 ;24938249 and the cytological breakpoints predicted from these coordinates are 98F1;98F10. Df(3R)BSC806 failed to complement Doa3 and Df(3R)Exel6211.