Isolation and characterization of Df(2L)BSC812
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC812 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f02709 and P{XP}d03477. The deletion was isolated as a chromosome carrying two copies of the miniwhite marker in progeny of P{hsFLP}1, y1 w1118; PBac{WH}f02709/P{XP}d03477 males crossed to w1118; P{hs-hid}2, wgSp-1/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP3.WH3}BSC812 from the segment of PBac{WH}f02709 to the left of its FRT site and the segment of P{XP}d03477 to the right of its FRT site. Exelixis, Inc. determined the insertion site of P{XP}d03477 to be at Release 3 genomic coordinate 13860811 on chromosome arm 2L. This corresponds to Release 5 coordinate  2L:13878659 . The insertion site of PBac{WH}f02709 is at Release 5 genomic coordinate 13421556 on arm 2L. Consequently, the breakpoints of Df(2L)BSC812 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  2L:13421556 ;13878659 and the cytological breakpoints predicted from these coordinates are 34B11;34E1.